Klebsiella

Klebsiella pneumoniae

Classification General Characteristics General characteristics Gram-negative bacilli Facultative anaerobes Non-motile Encapsulated (polysaccharide capsule) Single, in pairs, or short chains Form large, mucoid colonies Major pathogenic species: Klebsiella pneumoniae Biochemistry Lactose fermenters: Ferment lactose to lactic acid Pink colonies on MacConkey agar Urease positive: hydrolysis of urea into ammonia and carbon dioxide Oxidase negative Can use […]

Chlamydia

Chlamydial intranuclear inclusions

General Characteristics General characteristics Obligate intracellular bacteria: Cannot produce their own adenosine triphosphate (ATP) Capable of synthesizing their own macromolecules Staining: Visualized using Giemsa stain: nucleic acid stain Classified as gram-negative bacteria, but exhibit poor Gram staining  Cell wall shows some characteristics of gram-negative bacteria, but lacks peptidoglycans. Lack of peptidoglycans makes them insensitive to […]

Rickettsia

Giemsa-stained r. Rickettsii in the cells of a tick

General Characteristics General characteristics Transmission and geography Table: Summary of major clinically relevant species R. rickettsii R. prowazekii R. typhi R. akari Vector Hard ticks (Ixodidae family): Dermacentor (dog tick), Amblyoma (wood tick) Human lice (Pediculus humanus corporis): Defecate on skin Host scratches area. Bacteria enter the skin. Eastern flying squirrels along with their lice […]

Proteus

Proteus mirabili swarm

Classification General Characteristics Proteus spp. Clinically relevant species Epidemiology Pathogenesis Reservoir and transmission Virulence factors Disease process Clinical Presentation Diagnosis and Management Diagnosis Treatment Comparison with UTI-associated bacteria Escherichia coli (E. coli) and Proteus spp. are common etiologies of urinary tract infection. Table: Different factors of Proteus spp. versus E. coli Proteus spp. E. coli […]

Bacillus

Bacillus cereus

Classification General Characteristics Basic features of Bacillus spp. Pathogenic species B. anthracis Epidemiology Transmission Pathogenesis Clinical presentation In the United States, 3 clinical manifestations occur. Identification Management Prevention B. cereus Epidemiology Transmission Pathogenesis Clinical presentation Identification References

Yersinia pestis/Plague

3d computer generated image of yersinia enterocolita

General Characteristics Basic features of Yersinia pestis Structure: bacilli/rods Gram stain: gram negative  Other stain(s):  Stains such as Wright’s, Giemsa’s, and Wayson’s Bipolar staining (retain staining at the ends of the cells) or “safety pin” appearance  Spore formation: non-spore forming Invasion and replication in relation to the host cell(s): facultative intracellular Oxygen requirement: facultative anaerobes […]

Yersinia spp./Yersiniosis

3d computer generated image of yersinia enterocolita

Classification General Characteristics Basic features of Yersinia spp. Structure: bacilli/rods Gram stain: gram negative  Other stain(s):  Stains such as Wright’s, Giemsa’s, and Wayson’s Bipolar staining (retain staining at the ends of the cells) or “safety pin” appearance  Spore formation: non-spore forming Invasion and replication in relation to the host cell(s): facultative intracellular Oxygen requirement: aerobic, […]

Clostridia

Clostridium botulinum

Classification General Characteristics Basic features of Clostridium species Pathogenic species Table: Compare and contrast Clostridia species C. botulinum Clostridioides difficile C. perfringens C. tetani Habitat Soil  Marine sediments Intestinal flora Soil  Intestinal flora Soil  Intestinal flora Virulence Botulinum toxin Enterotoxin A Toxin B Alpha toxin Enterotoxin Tetanus toxin (tetanospasmin) Transmission Ingestion of: Spores Preformed toxin […]

Corynebacterium

Micrograph of corynebacterium diphtheriae

Classification General Characteristics Basic features General characteristics of Corynebacterium species include: Growth and biochemical properties Clinically relevant species Corynebacterium diphtheriae Epidemiology Transmission Virulence factors Clinical presentation Diphtheria (respiratory): Complications/systemic toxemia: Cutaneous (wound) diphtheria: Identification Prevention Management Mnemonic “ABCDEFGs of Corynebacterium diphtheriae”: References

Bordetella

Gram stain of b. Pertussis

Classification General Characteristics Basic features of Bordetella Biochemistry and growth Pathogenic species Bordetella Pertussis Epidemiology Transmission Pathophysiology Pathogenesis: Virulence factors: Clinical presentation Identification Specimen collection: Tests: Prevention References

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